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. 2019 Feb 4;9:1307. doi: 10.1038/s41598-018-37789-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of modified Vero cells and PEDVs. (A) MTT assay of Vero cells cultured at different concentrations of DSPE-PEG-DBCO for different hours. Each data point represents mean ± standard deviation (n = 9, P < 0.05) and was normalized to the Vero cells cultured without DSPE-PEG-DBCO in DMEM at the same time. (B) Titer of virus labeled with different concentrations of DBCO. Each data point represents mean ± standard deviation (n = 9, P < 0.05). (C) One-step growth curves of wild-type virus (WT-virus), DBCO labeled virus (DBCO-virus), and QD modified virus (QD-virus). Each data point represents mean ± standard deviation (n = 9, P < 0.05). (D) The emission spectra of QD-NH₂ (black), QD-N₃ (red) and QD-virus (blue).