Figure 4.

Validation of conduction velocity quantification methods in murine atria. (a) Activation maps from murine atria at 3 Hz and 10 Hz pacing (i), and during normoxia and hypoxia (ii). (b) Pacing at 10 Hz slows CV, measured using multi-vector method. (c) Representative activation curve showing prolongation of activation at 10 Hz vs 3 Hz pacing. (d) Pacing at 10 Hz slows CV, measured by time to 50% activation (act₅₀). (e) CV slowing induced by hypoxia, measured using multi-vector method. (f) Representative activation curve showing prolongation of activation curve by hypoxia. (g) Hypoxia slows CV, measured by time to 50% activation (act₅₀). Grouped data displayed as mean ± standard error, n = 8, *P < 0.05 by paired t-test.