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. 2019 Jan 18;10(2):50. doi: 10.1038/s41419-018-1298-y

Fig. 3. S1P1 increases the migration of (n)Tregs in BC microenvironments.

Fig. 3

a–c Migration of CD4+Foxp3+ cells in PBMCs after coculture with Biu87 and S1P or in medium with S1P (a), Biu87 cells transfected with lenti-shS1P1 vector, lenti-S1P1-expressing vector or the corresponding lenti-control vector (b), or Biu87 cells transfected with lenti-S1P1-expressing vector, lenti-S1P1-expressing vector and TGF-β antibody or with lenti-S1P1-expressing vector and IL-10 antibody (c) in a Transwell system for 24 h. Representative FACS staining data are shown. The statistical analysis were performed using the Student’s t-test. The bars represent the SEMs from three experiments. *P < 0.05, **P < 0.01