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. 2019 Feb 4;10(2):103. doi: 10.1038/s41419-019-1377-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The EMT pathway highlighted by GSEA analysis and the corresponding heatmap after CEMIP depletion in chondrocytes from five patients (left). RT-qPCR analysis of POSTN, SERPINE1, IL32, DKK1, and DKK2, genes in CEMIP-depleted chondrocytes compared to non-depleted chondrocytes. POSTN (n = 7), SERPINE1 (n = 8), IL32 (n = 7), DKK1 (n = 6), and DKK2 (n = 9) relative gene expressions were decreased in CEMIP-depleted cells (right). (ANOVA paired tests: *p < 0.05, **p < 0.01, ***p < 0.001). b ELISA analysis of Periostin (left) and DKK1 (right) secretion in CEMIP-depleted cells and control cells. Secretions of Periostin (n = 10) and DKK1 (n = 10) were decreased in CEMIP-depleted chondrocytes compared to control chondrocytes. (ANOVA paired tests: *p < 0.05, **p < 0.01, ***p < 0.001). c RT-qPCR analysis of CTNB1 and BTRCP genes in CEMIP-depleted chondrocytes compared to non-depleted chondrocytes (up). BTRCP (n = 8) was increased in CEMIP-depleted cells compared to control cells while CTNB1 (n = 9) expression was not modified (ANOVA paired tests: *p < 0.05). Representative picture of western blot analysis of β-catenin, β-TRCP, CEMIP, and HSP90 expression in cells treated with shEGFP, shCEMIP#1, and shCEMIP#2 (left). Analysis was done on several patients: western blot quantification illustrating the decrease of β-catenin/HSP90 expression (n = 5) and the increase of β-TRCP/HSP90 (n = 8) in cells treated with shCEMIP#1 and #2 compared to shEGFP treated cells (bottom). (ANOVA paired tests: *p < 0.05, **p < 0.01)