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. 2019 Feb 4;9:1325. doi: 10.1038/s41598-018-37394-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison between tomograms of unstained and stained lungs. The staining procedure with I2E increased the image contrast, as is seen in the comparison of the tomograms of (A) an unstained sample and of (B) a lung stained with I2E. Details are better seen in the enlarged images of the areas selected with a square, shown below the corresponding sample. Arrows indicate lobe bronchi and small airways.