Table 1.

Tumor burden calculation performed for different samples using three different methods: (1) from the tumor area segmented manually in 2D images of H&E-stained specimens; (2) from the tumor area segmented using a semi-automated method and micro-CT slices of I2E-stained specimen; and (3) and tumor volume reported as the sum of measurements of all the tumorous areas of all virtual micro-CT slices.

Sample	Tumor burden (%)
	H&E histological slice	micro-CT virtual slice	micro-CT volumetric image
1a	6.9	7.6	16.9
2b	5.9	—	20.7
3c	20.5	22.8	65.8
4d	3.3	10.5	8.8

Columns from left to right: ^aFigs 3D and 4A,D and ; ^bFigs 3E and 4B,E; ^cFigs 3F and 4C,F (respectively equal to Fig. 5A,C); ^dFig. 5B,D, and micro-CT volumetric image not shown.