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. 2019 Feb 4;9:1335. doi: 10.1038/s41598-019-38579-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cells overexpressing CDC25 are sensitive to CDC25 inhibitors. (A) U2OS Tet-OFF cells expressing HA-CDC25A were treated with the indicated compounds and cell viability was determined. P-values were determined by non-linear regression analysis and curve fitting comparison using the extra sum-of-squares F-test. (B) Western blot (WB) analysis of HA-CDC25A expression in the presence or absence of tetracycline. PR: Ponceau Red staining.