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. 2019 Feb 4;9:1335. doi: 10.1038/s41598-019-38579-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CDC25 inhibitors block the growth of K-Ras-dependent intestinal organoids. (A) Organoids reproducing duodenal crypts and derived from Apc/K-Ras mouse small intestine (left) were grown in the presence of UPD-176 (right). Phase contrast stills were obtained at 48 h of treatment. (B) Confocal immunofluorescence microscopy of Apc/K-Ras organoids treated as in A and stained for proliferation (β-catenin) or differentiation (lysozyme) markers. DNA was stained with DAPI. (C) qRT-PCR on cDNA obtained from control- and UPD-176-treated organoids was conducted using primers to the indicated markers.