Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 4;10(2):104. doi: 10.1038/s41419-018-1200-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Western blot detection of DDB1 and Cul4A in A2780CP cells transduced with virus containing shRNA against DDB1 or Cul4A, respectively, or scrambled shRNA (non-target control, NT). GAPDH serves as loading control. b RT-PCR of DDB1 and Cul4A expression in A2780CP cells transduced with virus containing shRNA against DDB1 or Cul4A, respectively, or scrambled shRNA (NT). Data represent mean ± SD normalized to actin. Results were averaged from three independent experiments, measured in triplicate. Significance of differences was calculated using Student’s t test (**P < 0.01, ****P < 0.0001). c Flow cytometry analysis showing cell cycle distribution of A2780CP cells transduced with virus containing shRNA against DDB1 or Cul4A, or scrambled shRNA (NT). Quantification data were averaged from three independent experiments. Data represents mean ± SD. Significance of differences was calculated using Student’s t test (*P < 0.05)