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. 2019 Feb 4;10(2):104. doi: 10.1038/s41419-018-1200-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Venn diagram showing gene expression profile overlap between A2780CP cells with Cul4A knockdown (left circle) and cells wtih DDB1 knockdown (right circle). Total RNA was extracted from cells with scrambled shRNA or with either Cul4A or DDB1 knockdown, and the transcriptome was analyzed with sequencing. b Heatmap representing unsupervised hierarchical clustering of mRNA expression level in A2780CP cells transfected with control (NT) or CRL4 (Cul4A/DDB1) shRNA. Each column represents the indicated sample, and each row indicates the mRNA level of one gene. Red and green colors indicate high and low expression, respectively. c RT-PCR of BIRC3 expression in A2780CP cells transduced with virus containing scrambled shRNA (NT) or shRNA against DDB1/Cul4A. Data represent mean ± SD normalized to actin. Results were averaged from three independent experiments, measured in triplicate. Significance of differences was calculated using Student’s t test (****P < 0.0001). d Western blot detection of DDB1, Cul4A, and BIRC3 in A2780CP cells transduced with virus containing scrambled shRNA (NT) or shRNA against DDB1/Cul4A. GAPDH serves as loading control. e The overall survival rates of ovarian cancer patients (from TCGA database) were compared between the BIRC3-low and BIRC3-high groups. Statistical significance was determined using the log-rank test (P < 0.05)