Table 4.
Storage effect on particle-size, zeta-potential, polydispersity, encapsulation, loading and cumulative drug release of PLGA-NPs for 3 months at 25 °C (mean ± SD, n = 3).
| At 3 months storage | Particle-size (nm) | Polydispersity index | Zeta-potential (mV) | Encapsulation (% EE) | ACY loading (% DL) | Cumulative release of ACY (%) |
|---|---|---|---|---|---|---|
| Initially | ||||||
| PLGA-NPs-PVP (F2) | 174.46 ± 15.25 | 0.297 ± 0.015 | −15.82 ± 3.69 | 60.25 ± 4.92 | 6.75 ± 1.32 | 64.67 ± 4.29 |
| PLGA-NPs-TPGS (F5) | 262.38 ± 11.85 | 0.255 ± 0.011 | +15.14 ± 2.81 | 74.12 ± 6.19 | 8.65 ± 1.09 | 66.99 ± 4.78 |
| After 1 week | ||||||
| PLGA-NPs-PVP (F2) | 187.25 ± 9.25 | 0.302 ± 0.014 | −16.25 ± 2.74 | 60.11 ± 2.75 | 6.25 ± 1.15 | 63.75 ± 2.34 |
| PLGA-NPs-TPGS (F5) | 271.15 ± 8.51 | 0.261 ± 0.017 | +15.35 ± 1.98 | 73.75 ± 3.05 | 7.99 ± 1.24 | 66.38 ± 2.18 |
| After 1 month | ||||||
| PLGA-NPs-PVP (F2) | 205.32 ± 11.37 | 0.312 ± 0.025 | −15.23 ± 2.11 | 60.01 ± 1.87 | 6.19 ± 1.07 | 61.25 ± 1.75 |
| PLGA-NPs-TPGS (F5) | 302.25 ± 9.75 | 0.293 ± 0.031 | +16.27 ± 2.43 | 61.26 ± 1.24 | 7.81 ± 1.14 | 65.58 ± 1.86 |
| After 3 months | ||||||
| PLGA-NPs-PVP (F2) | 273.18 ± 12.43 | 0.345 ± 0.027 | −17.26 ± 2.17 | 58.23 ± 1.19 | 6.03 ± 1.02 | 60.12 ± 1.25 |
| PLGA-NPs-TPGS (F5) | 354.63 ± 15.45 | 0.312 ± 0.008 | +17.25 ± 2.08 | 60.34 ± 1.75 | 7.79 ± 1.05 | 63.78 ± 1.97 |