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. 2019 Feb 4;9:1302. doi: 10.1038/s41598-018-37690-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qRT-PCR and Western blot results at week 9. Left ventricular (a) miR-212 expression, (b) total AKT expression, (c) phospho-AKT expression, (d) phospho-AKT/total AKT ratio, (e) FOXO3 mRNA expression, (f) total FOXO3 expression, (g) phospho-FOXO3 expression, and (h) phospho-FOXO3/total FOXO3 ratio. White bars represent the sham-operated group, and grey bars represent the chronic kidney disease (CKD) group. Values are means ± SEM, n = 7–8, *p < 0.05, unpaired t-test (a–e) or Mann-Whitney-U test (f–h).