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. 2019 Feb 4;9:1395. doi: 10.1038/s41598-019-38554-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) In vitro experimental setup, (b) cytotoxicity assay of experimental groups: control (no treatment), cell culture media with ultrasonic treatment; DMSO only; verteporfin added but non-treated; expectation (verteporfin introduced cell culture media with active μLights by ultrasonic treatment); and Triton X100 treated (added to kill cells), (c) in-vivo experimental setup: μLight was implanted in mouse and excited/powered via ultrasound, (d) optical image of the mouse (24 hours after PDT treatment), (e) Tumor volume change with respect to the size when device was implanted. PDT treatment with μLights was conducted 7 days after the surgery allowing for wound healing.