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. 2019 Feb 4;9:1262. doi: 10.1038/s41598-018-37766-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inner mural granulosa cells send projections in many directions. (A) SEM image showing multiple inner mural granulosa cell bodies in cross-section. Four cells (colored) were chosen for reconstruction. Scale bar, 5 μm. Video 6 shows 240 serial sections of these cells and their projections. (B) Reconstruction of 4 inner mural granulosa cell bodies from (A) and every cytoplasmic projection derived from them. Reconstruction is 21.2 × 16.4 × 20.8 μm (x, y, z), encompassing 462 serial sections (each, 45 nm-thick). A rotating view of this reconstruction can be seen in video 7. (C) Front and side views of individual inner mural granulosa cell reconstructions from (B).