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. 2019 Feb 4;9:1263. doi: 10.1038/s41598-018-37419-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Astrocytic endfeet re-cover blood vessels regardless of life or death of ablated astrocyte and position of the laser ablation. Laser ablation on an endfoot (A) and a stalk of process (B) of astrocytes. Astrocytic endfoot covering a blood vessel (Aa) disappeared 40 min after the laser ablation (filled arrowhead in Ab), and another endfoot of the same astrocyte re-covered the blood vessel 90 min after (arrow in Ac). (B) Laser ablation on a stalk of astrocytic process. The endfoot covering a blood vessel (Ba) disappeared 30 min after the laser ablation (filled arrowhead in Bb) and re-covered the blood vessel 22 h after (arrow in Bc). Open arrowheads point cell bodies of the ablated astrocytes. Dotted line, locations of laser ablation. Scale bar, 10 µm. These images were constructed by maximum intensity z-projections of 30–50 (A) and 20–35 µm (B) depth from the brain surface.