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. 2019 Feb 4;9:1263. doi: 10.1038/s41598-018-37419-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic drawings of laser ablation of astrocytic endfeet showing patterns of astrocytic endfeet behavior after laser ablation. (A) Re-covering of stripped blood vessels irrespective of death (Aa) or life (Ab) of ablated astrocytes. (B) Stripped blood vessels were re-covered by the endfeet of ablated astrocytes (Ba) or those of other astrocytes (Bb). (C) Either endfeet (Ca) or stalks of endfeet (Cb) were laser ablated. Ablated astrocytes are colored in bright green, other astrocytes in dark green and blood vessels in red. Lightning symbols and dotted lines represent laser ablated loci, and arrowheads show removed astrocytic endfeet by laser ablation.