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. 2019 Feb 4;9:1295. doi: 10.1038/s41598-018-37686-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell density effects on YAP expression from hiPSCs. (A) Representative fluorescent images of markers α-actinin and YAP for different seeding densities (1 × 10⁴, 2 × 10⁴, 5 × 10⁴ initial cells/spheroid in each well of 96-well plate) at day 18. Scale bar: 100 μm. (B) The quantitative analysis of nuclear and cytoplasmic YAP expression. *Indicates p < 0.05.