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. 2019 Feb 4;9:1332. doi: 10.1038/s41598-018-37971-z

Table 4.

Primers used to generate DNA probes specific to the IR of TYLCSV and TYLCV.

Begomovirus [isolate] GenBank acc. number Primers (5′-3′) Probe coordinates* Reference
TYLCSV-ES [ES-Mur1-92] Z25751 MA-14 (TGCATTTATTTGAAAACG)
MA-15 (AAAGGATCCCACATATTG)
2580–155 25
TYLCV-Mld [ES-72-97] AF071228 MA-30 (GAGCACTTAGGATATGTGAGG)
MA-31 (AGTGGATCCCACATATTGC)
2557–161 25

*Nucleotide coordinates considering as first nucleotide that after the nicking site within the conserved nonanucleotide at the origin of replication.