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. 2019 Feb 4;9:1177. doi: 10.1038/s41598-018-38018-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Clostridium ramosum on lipid metabolism in epididymal white adipose tissue (eWAT) of mice fed HFD. (A) mRNA expression of Cd36, Fatp1, Fatp4, adipocyte Protein 2 (aP2) and Plin2 in eWAT of Cra and GF mice fed either LFD or HFD. (B,C) mRNA expression of PPAR-α, Pparɣ, carnitine palmitoyl transferase I (Cpt1a), adipose triglyceride lipase (Atgl) and hormone sensitive lipase (Hsl) in eWAT of indicated mice. (D) Representative Western blots of eWAT-derived proteins with antibodies specific for p-ACC1 (280 kDa), total ACC, p-HSL (81.83 kDa) or total HSL. MITOFUSIN-2 (MFN-2) (86 kDa) was used as a loading control. (E,F) mRNA and protein abundance of ATP citrate lyase (ACLY) (125 kDa) and fatty acid synthase (FASN) (273 kDa) in eWAT of indicated mice. MITOFUSIN-2 (MFN-2) (86 kDa) was used as a loading control. (A–E) Bars indicate mean ± SEM log2 fold change compared to mean of LFD-fed GF group. Asterisks above bars indicate statistical differences between Cra and GF mice for each diet group analyzed by Mann-Whitney U test. Differences among all 4 groups were analyzed by Kruskal-Wallis and Dunn’s Post-hoc tests and are indicated by lines. n = 8–13. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. See also Fig. S5.