Skip to main content
. 2019 Feb 4;9:1350. doi: 10.1038/s41598-018-38014-3

Figure 2.

Figure 2

ApoA-I phosphorylates IRβ, IRS1, Akt and AS160 in HSKMCs. HSKMCs were pre-incubated at 37 °C for 16 h in serum-free MEM-α with or without lipid-free apoA-I then incubated with insulin as described in the legend to Fig. 1. The cells were lysed, subjected to SDS-PAGE and immunoblotted for (A) total and phosphorylated IRβ, (B) total and phosphorylated IRS-1, (C) total and phosphorylated Akt, (D) total and phosphorylated AS160 using β-actin as the loading control. Values represent the mean ± SD of three independent experiments.*p < 0.05, **p < 0.01, ***p < 0.001 vs. control (open bars).