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. 2019 Feb 4;9:1350. doi: 10.1038/s41598-018-38014-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ApoA-I increases insulin-dependent glucose uptake in HSKMCS in an IRS-1 and PI3K/Akt/AS160-dependent manner. (A) HSKMCs were transfected with scrambled siRNA (scr siRNA) or IRS-1 siRNA. IRS-1 protein expression was quantified by immunoblotting. (B) Scr-siRNA- (open bars) and IRS-1 siRNA-transfected (closed bars) HSKMCs were pre-incubated with or without apoA-I then incubated in the presence or absence of insulin. Glucose uptake was quantified as described in the legend to Fig. 1. (C–E) Total and phosphorylated Akt, and AS160 were determined by immunoblotting after incubation in the presence (closed bars) and absence (open bars) of wortmannin. (F) Glucose uptake in HSKMCs was determined in the presence (closed bars) and absence (open bars) of wortmannin as described in Materials and Methods. All values represent the mean ± SD of three independent experiments. Glucose uptake was normalized to 1 nmol/mg/h for control scr siRNA-transfected cells. ***p < 0.001 vs. control for scr siRNA-transfected cells or cells incubated in the absence of wortmannin; ^†p < 0.05 versus control IRS-1 siRNA-transfected cells; ^†††p < 0.001 versus cells incubated with wortmannin under control conditions.