Figure 4.

ApoA-I increases GLUT4 translocation to the HSKMC plasma membrane. HSKMCs were pre-incubated at 37 °C for 16 h with or without apoA-I then incubated in the presence and absence of insulin as described in the legend to Fig. 1. (A) Cell surface GLUT4 was labeled with an antibody that specifically recognizes the extracellular domain of the protein and analysed by flow cytometry. (B) Total cell lysates were immunoblotted for GLUT4 using β-actin as a loading control. (C) GLUT4 mRNA levels were quantified by real-time PCR. Values represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ****p < 0.0001 vs. control (open bar).