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. 2019 Feb 4;10:562. doi: 10.1038/s41467-019-08421-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Invadopodia are cell autonomously required for spinal entry. a Confocal z-projection images taken from a 24-h time-lapse starting at 48 h post fertilization (hpf) of a Tg(sox10:lifeact-GFP); Tg(sox10:PA-Rac1-mcherry) zebrafish with both photoactivated and non-photoactivated Rac1. Orange box denotes dorsal root entry zone (DREZ). White arrow denotes growth cone. b Quantification of the number of PA-Rac1⁺ neurons that enter the spinal cord. Photoconverted neurons fail to enter the spinal cord. SEM is shown, n = 10 non-photoactivated dorsal root ganglia (DRG), n = 9 photoactivated DRG. c Quantification of the number of PA-Rac1⁺ neurons that form actin-invasive structures. Photoactivated neurons fail to form actin-invasive components. SEM is shown, n = 10 DRG. d Intensity profiles of growth cone Lifeact-GFP in neurons with photoactivated (n = 9) and non-photoactivated (n = 10) Rac-1. e Distance traveled by neurons with photoactivated (n = 9) and non-photoactivated (n = 10) Rac-1. f Confocal of two growth cones of a Tg(sox10:lifeact-GFP) animals stained for Cortactin showing co-localization of Cortactin with Lifeact-GFP foci at the DREZ. g Confocal images of a Tg(ngn1:gfp) animals stained for pSrc showing localization of pSrc in pioneer growth cones. Scale bars denote 10 µm in (a) and 5 µm in (f, g). Insets in (f, g) are single-plane confocal images