Fig. 7.

Entry defects from failed invasion are rescued by dorsal root entry zone (DREZ) lesioning. a Confocal z-projection frames from a 24-h time-lapse starting at 48 h post fertilization (hpf) of Tg(gfap:gfp), Tg(sox10:lifeact-gfp) zebrafish treated with SU6656 with and without a DREZ lesion showing pioneer axon navigation. b Quantification of pioneer axon entry in animals treated with SU6656 and a lesioned DREZ (n = 12), SU6656 without a lesioned DREZ (n = 9), SU6656 (n = 20), and dimethyl sulfoxide (DMSO; n = 14). SEM is shown. c Quantification of Tg(tnfa:gfp)⁺ dorsal root ganglia (DRG) in animals treated with DMSO without a DREZ lesion (n = 30), animals treated with SU6656 without a DREZ lesion (n = 30), and animals treated with SU6656 with a DREZ lesion (n = 28). d Schematic representation of actin-rich invasion that comprises Cajal’s battering ram. Scale bars denote 10 µm