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. 2019 Feb 4;9:1256. doi: 10.1038/s41598-018-37628-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Macrophage ROS production in the presence of extracts from BC dressings impregnated with PEG - PC; clove oil - C, eucalyptus oil - E, thyme oil - T. SP - spontaneous ROS production by non-treated macrophages. (b) Visualization of macrophage ROS: 1. spontaneous (non-treated) production; 2. LPS-induced ROS production; 3. ROS production induced by extract from BC dressing impregnated with eucalyptus essential oil. Green color - ROS production; blue color - macrophages’ nuclei. Please note that intensity of fluorescence of EO-treated macrophages (at green channel, especially) is similar to this of control sample and lower than of LPS-induced production. Control set: Results are normalized with regard to CP sample which consists of cellulose and oil PEG but does not possess active substances of EOs (values of ROS production for CP was considered 100%).