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. 2019 Feb 4;9:1235. doi: 10.1038/s41598-018-37878-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dendritic cells (DCs) differentiated with betamethasone impair autologous γδ⁺ T lymphocyte proliferation. DCs were loaded with insulin (20 µg/ml) and cultured with splenocytes in a ratio 1:10 for 4 days. White circles represent immature DCs (iDCs), black squares represent mature DCs (mDCs) generated in basal conditions and stimulated with lipopolysaccharide (LPS), grey symbols represent mDCs with 10 nM (triangles), 100 nM (dots) and 1000 nM (rhombus) betamethasone (bet) during differentiation and stimulated with LPS. (A) Percentage of γδ⁺ T cells within CD3⁺ population after co-culture with DCs. (B) Percentage of autologous proliferation of γδ⁺ T cells (% CFSE^low) after co-culture with DCs. (C) IL-17A production in T cell proliferation experiments measured in supernatants after 4 days of co-culture of DCs and splenocytes. Lines show the mean of 5 independent experiments (*p ≤ 0.05, **p < 0.01, Dunn’s test, Kruskal-Wallis test).