Figure 2.

CD4⁺ T cells expansion after neutrophils influx to dLNs. Flow cytometry analysis of cell populations in dLNs and ndLNs obtained from immunized mice at different time points after footpad injection. In the dotted line, normal values of LNs from unimmunized mice are shown as reference. Absolute number of (A) total cells; (B) CD4⁺ T cells; (C) CD62L⁺CD44⁻ naïve, CD62L⁻ CD44⁺ effector memory, and CD62L⁺ CD44⁺ central memory CD4⁺ T cells. Results are representative of three independent experiments and are expressed as mean ± SEM (n = 4/group); ns, not significant, *p < 0.05, **p < 0.001, ***p < 0.001.