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Mutant p53 interferes with MDM2 RING function. (a) MDM2 or MDM2ΔAD (with deletion of residues 210 to 290) was coexpressed with mutant p53 and His6-ubiquitin in H1299 cells. MDM2 self-ubiquitination was analyzed by Ni2+-NTA pulldown and MDM2 Western blotting. (b) MDM2 or MDM2-Praja (MDM2 RING replaced with Praja RING) was cotransfected with p53 and His6-ubiquitin in H1299 cells. MDM2 self-ubiquitination was analyzed by Ni2+-NTA pulldown and MDM2 Western blotting.
