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. 2018 Dec 17;20(2):e46429. doi: 10.15252/embr.201846429

Figure EV2. qPCR expression levels of RNA samples in different brain regions and ages of miR379‐410 wt/ko mice.

Figure EV2

  1. Expression of precursor miRNAs in hippocampal neonatal (P1) miR379‐410 wt/ko pups: pre‐miR‐124 (wt n = 5, ko n = 5, t 8 = 0.5848, ns P = 0.5748), pre‐miR‐132 (wt n = 5, ko n = 5, t 8 = 0.8740, ns P = 0.4076), pre‐miR‐137 (wt n = 5, ko n = 5, t 8 = 5.037, **P = 0.0010), pre‐miR‐138.2 (wt n = 5, ko n = 5, t 8 = 0.4145, ns P = 0.6894) and from the miR379‐410 cluster pre‐miR‐134 (wt n = 5, ko n = 5, t 8 = 7.637, ****P < 0.0001), unpaired Student's t‐test.
  2. Expression of precursor miRNAs in hippocampal juvenile (P14) miR379‐410 wt/ko mice: pre‐miR‐124 (wt n = 4, ko n = 4, t 6 = 1.406, ns P = 0.2093), pre‐miR‐132 (wt n = 4, ko n = 4, t 6 = 0.5348, ns P = 0.6120), pre‐miR‐137 (wt n = 4, ko n = 4, t 6 = 1.250, ns P = 0.2578), pre‐miR‐138.2 (wt n = 4, ko n = 3, t 5 = 0.9339, ns P = 0.3932) and from the miR379‐410 cluster pre‐miR‐134 (wt n = 4, ko n = 4, t 6 = 3.150, *P = 0.0198); unpaired Student's t‐test.
  3. Expression of mature miRNAs in cerebellum of adult miR379‐410 wt/ko mice: miR‐124 (wt n = 5, ko n = 4, t 7 = 1.214, ns P = 0.2642), miR‐132 (wt n = 5, ko n = 4, t 7 = 1.382, ns P = 0.2095), miR‐138 (wt n = 5, ko n = 4, t 7 = 1.089, ns P = 0.3120) and from the miR379‐410 cluster miR‐329 (wt n = 3, ko n = 2, t 3 = 7.720, **P = 0.0045); unpaired Student's t‐test.
  4. Expression of precursor miRNAs in cerebellum of adult miR379‐410 wt/ko mice: pre‐miR‐124 (wt n = 3, ko n = 2, t 3 = 0.8014, ns P = 0.4815), pre‐miR‐137 (wt n = 3, ko n = 2, t 3 = 0.8107, ns P = 0.4769), pre‐miR‐138.2 (wt n = 3, ko n = 2, t 3 = 0.5169, ns P = 0.6409) and from the miR379‐410 cluster pre‐miR‐369 (wt n = 3, ko n = 2, t 3 = 16.78, ***P = 0.0005); unpaired Student's t‐test. Data are presented as mean ± s.e.m.