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. 2019 Jan 8;20(2):e46612. doi: 10.15252/embr.201846612

Figure EV3. SPRR5 transcript characterization.

Figure EV3

  1. Schematic overview of the genomic localization of SPRR5.
  2. Exact genomic coordinates for the verified SPRR5 transcript (genome release: hg38) and exemplary pictures of aligned and mapped RNA‐Seq reads for control (siCtrl) and SPRR5 knockdown (siSPRR5) with respect to LINC01527 (lower part). The top part indicates the localization of the utilized probes for northern blot mapping as well as the results from hybridization of membranes with RNA from differentiated keratinocytes. Furthermore, the verified transcript and the Ensembl annotated version are shown and red stars indicate the RACE (rapid amplification of cDNA ends) verification of start and end points of the transcript.
  3. Upstream transcription factor predictions for SPRR5 obtained by ARCHS4.
  4. UCSC view showing the genomic locus of SPRR5 as well as the p63 ChIP‐Seq peaks from studies by Kouwenhoven et al and Bao et al.
  5. Knockdown of LINC00941 does not affect pan‐p63 levels in human epidermal tissue (n = 3–4 epidermal tissue cultures/knockdown group). Data are presented as mean ± SD.