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. 2018 Dec 10;20(2):e46221. doi: 10.15252/embr.201846221

Figure 2. Small intestinal fetal organoids recapitulate suckling‐to‐weaning transition in vitro .

Figure 2

  • A–G
    Real‐time qPCR analysis of fetal organoids cultured for 1 month showing decrease in relative expression of indicated neonatal markers (A) Ass1, (B) Blimp‐1, (C) FcRn, and (D) Lct and increase in the adult markers (E) Sis, (F) Treh, and (G) Arg2 (n = 3 individual wells from single organoid culture (see Materials and Methods); experiment was repeated in four to eight independent organoid cultures with similar results).
  • H–L
    Enzyme activity assay of fetal organoids for (H) lactase, (I) sucrase, (J) maltase, (K) trehalase, and (L) arginase. Activity is given in μM glucose/μg protein/min (experiment was generated from single organoid culture (see Materials and Methods) and repeated in three independent organoid cultures with similar results).
Data information: Data are presented as mean ± SEM. ND not detected, *P < 0.05, **P < 0.01, ***P < 0.001, NS: not significant, relative to expression or activity level at day 3 (one‐way ANOVA).