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. 2018 Dec 10;20(2):e46221. doi: 10.15252/embr.201846221

Figure 3. Fetal organoids resemble adult organoids after 1 month in culture.

Figure 3

  • A–G
    Relative expression detected by real‐time qPCR in fetal organoids (black circles) and adult organoids (blue triangles). Neonatal markers (A) Ass1, (B) Blimp‐1, (C) FcRn, and (D) Lct in fetal organoids cultured for 1 month compared to levels adult organoids. Mature markers (E) Sis, (F) Treh, and (G) Arg2 increase in fetal organoids to adult levels (n = 3 individual wells from single organoid culture (see Materials and Methods); experiment was repeated in four independent organoid cultures with similar results).
  • H–L
    Enzyme activity assay of fetal (black bars) and adult (blue bars) organoids. (H) Lactase activity is higher in fetal organoids decreasing to adult levels at day 28, while (I) sucrase, (J) maltase, (K) trehalase, and (L) arginase increase to adult levels. Activity is given in μM glucose/μg protein/min (n = 3 independent organoid cultures).
Data information: Data are presented as mean ± SEM. ND not detected, *P < 0.05, **P < 0.01, ***P < 0.001, NS: not significant, in (A–G) between fetal and adult organoids and in (H–L) relative to adult levels (t‐test).