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. 2018 Dec 10;20(2):e46221. doi: 10.15252/embr.201846221

Figure EV4. Fetal organoids mature in vitro .

Figure EV4

  • A–D
    Real‐time qPCR analysis of fetal organoids cultured for 1 month showing decrease in relative expression of (A) CRAMP and increase in the Paneth cell markers (B) Lyz1, (C) Defcr1, and (D) Defcr5 (n = 3 individual wells from single organoid culture (see Materials and Methods); experiment was repeated four times with similar results).
  • E
    Microscopic images of fetal and adult organoids at days 6, 17, and 28 of culture. Scale bars: 250 μm.
  • F–H
    Real‐time qPCR of (F) Villin1, (G) ChgA, and (H) Muc2 (n = 3 individual wells from single organoid culture (see Materials and Methods); experiment was repeated four times with similar results).
Data information: Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, NS: not significant, relative to expression level at day 3 (one‐way ANOVA).