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. 2018 Dec 10;20(2):e46221. doi: 10.15252/embr.201846221

Figure 5. Spheroids and organoids show the same protein expression pattern, independently of culture stage/passage.

Figure 5

  • A
    Microscopic images of fetal organoids at days 3, 13, 20, and 30 of culture. Scale bars: 500 μm.
  • B
    Percentage of fetal spheroids decreases during culture (n = 3 individual wells from single organoid culture (see Materials and Methods); experiment was repeated in ten independent organoid cultures with similar results).
  • C–E
    Immunohistochemistry of fetal organoids for (C) Ass1, (D) Sis, and (E) Arg2. Quantification of three slides per staining, 7–10 organoids/spheroids per slide. Scale bars: 50 μm.
Data information: Data are presented as mean ± SEM. ***P < 0.001, relative to number of spheroids at day 3 (one‐way ANOVA).