Figure 6. TCGA SpliceSeq analysis using BRCA dataset in ESRP1 _high and ESRP1 _low tumors.

1. The data were analyzed by categorizing the splice events into seven types: exon skip (ES), alternative 5′ donor (AD), alternative 3′ acceptor (AA), retained intron (RI), mutually exclusive exons (ME), alternative first exon (AP), and alternative last exon (AT). The analysis of splice events was performed using the following filter criteria: Min Gene RPKM ≥ 2, |dPSI| ≥ 0.1, P ≤ 0.02, Min Group Obs % > 0.85; RPKM‐reads per kilobase of transcript per million aligned reads; and |dPSI|‐absolute changes in percent splicing (dPSI, ∆Ψ). The events are presented as differential expression of splice events between ESRP1_low and ESRP1_high cases.
2. TCGA splice graphs for the EMT signature genes in ESRP1_high versus ESRP1_low tumors. A splice graph of the gene's exons is shaded based on the expression level and shows the selected splice event outlined in red.

Source data are available online for this figure.