Binary food choice assays were conducted after GRNs were ablated by expressing the cell death gene (hid), under Gr28b.c‐GAL4 (n = 4).
Tip recording analysis of the indicated S‐type sensilla and genotypes (n ≥ 7).
Expression of the Gr28b.c‐GAL4 reporter in subsets of Gr66a‐positive GRNs. First panel shows that all bitter‐responsive GRNs are labeled by Gr66a‐I‐GFP (anti‐GFP, green). Second panel shows the expression of the Gr28b.c‐GAL4, crossed with UAS‐DsRed (anti‐DsRed, red). Third panel shows merged image of first and second panels. Forth panel shows a schematic diagram of sensilla arrangement. Red‐marked sensilla indicate the expression of Gr28b.c‐GAL4 reporter in second panel. Scale bars represent 40 μm.
Data information: The error bars represent SEMs. The asterisks indicate significant differences from that of the control detected by a single‐factor ANOVA with Scheffe's analysis as a
post hoc test to compare two sets of data (**
P < 0.01).