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. 2019 Jan 25;9:3169. doi: 10.3389/fimmu.2018.03169

Figure 1.

Figure 1

Optimisation of the NK:CTV-1 priming ratio using NK cells from healthy volunteers. NK cells were isolated from thawed PBMCs that had previously been generated from the peripheral blood of healthy individuals and co-incubated with mitomycin C treated CTV-1 cells at ratios 2:1, 1:1, 1:2, 1:4 for 17 h at 37°C. NK cells incubated in isolation were used as controls. The phenotype and cytotoxic function of the primed NK cells were then assessed by flow cytometry. For each priming ratio, the ability of primed NK cells to lyse K562 cells and PC3 cells at a 5:1 effector to target ratio were measured. (A–C) Cytotoxicity against K562 and PC3 cells for three healthy volunteers. (D,E) Significant correlation between K562 lysis and expression of activating receptors NKG2D and NKp46 on primed NK cells, respectively.