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. 2019 Jan 28;10:83. doi: 10.3389/fmicb.2019.00083

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Copyright © 2019 Shao, Tang, Chen, Wu, Wang, Ma, Zhou, Chen and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis and expression profiles of Dicers, RDRPs, and AGOs in C. militaris and other fungi. Phylogenetic trees constructed with fungal Dicer (A), RDRP (B), and AGO (C) proteins. CM, Cordyceps militaris; BB, Beauveria bassiana; MA, Metarhizium robertsii; OS, Ophiocordyceps sinensis; MO, Magnaporthe oryzae; FG, Fusarium graminearum; CP, Cryphonectria parasitica; NC, Neurospora crassa; AF, Aspergillus flavus; AT, Arabidopsis thaliana. Expression profiles of Dicers, RDRPs, and AGOs at different developmental stages of C. militaris (D). Total RNAs were extracted at different asexual developmental stages of C. militaris, i.e., 3-day-old (A3), 9-day-old (A9), and 15-day-old (A15), and from different sexual developmental stages, i.e., nascent (SNF), stalk formation (SMIF), and mature fruiting bodies (SMAF). Data are expressed as means ± SE of the values from six independent experiments. Student’s t-test was used to determine the statistical significance of differences between groups. The asterisk “^∗” represents a significant difference level of P < 0.05 compared with A3 (asexual development) or SNF (sexual development).