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. Author manuscript; available in PMC: 2019 Feb 5.
Published in final edited form as: J Am Chem Soc. 2017 Dec 1;139(49):17861–17869. doi: 10.1021/jacs.7b08465

Figure 6. Solubility of P2-Insulin Affected by pH and Photolysis.

Figure 6.

(a) A 75µM solution of P2-Insulin in 10mM pH 4 PBS. (b) Solution becomes cloudy at point of entry of NaOH that shifts pH to the neutral. (c) After vortexing, material is now at pH 7.2 and is a suspension. (d) After photolysis for 10 minutes at 365nm, the solution is partially clarified. (e) After suspension is centrifuged to clarify, clear supernatant removed, additional pH 7.2 buffer added, and further irradiation at 365nm takes place, material is completely clear.