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. 2019 Jan 22;2019:5018410. doi: 10.1155/2019/5018410

Figure 3.

Figure 3

Effects of BCL6 knockdown on Ang II-induced VSMC proliferation and oxidative stress in VSMCs. The cells were treated with Scr-shRNA or BCL6 shRNA (1 μg/mL) for 24 h followed by treatment with PBS or Ang II (100 nM) for 48 h. (a) VSMC proliferation determined by CCK-8 assay. (b) PCNA protein expression. (c) NOX2 and NOX4 protein expressions. (d) ROS detected by dihydroethidium (DHE) staining. (e) Bar graph showing the relative fluorescence intensity of DHE. (f) BCL6 protein expression. Values are mean ± SE. ∗P < 0.05 vs. PBS; †P < 0.05 vs. Ctrl. n = 6 for each group.