Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Experimental design of hiPSC expansion. The QES hollow fibers were coated with LN521 or VN for at least 4 hours. Human iPSCs were loaded in the QES on day 0 in a density of >35 × 106 cells in 100 ml of TeSR™ E8™ media with 10 μM Y-27632. Cells were fed via the media inlet line from days 1–6/7, at which time the cells were harvested, frozen, and/or characterized for pluripotency and differentiation markers.
