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Infection of HeLa cell monolayers following C. trachomatis release from biofilms. Infectivity of C. trachomatis EBs released from C. albicans (a) or G. vaginalis (b) biofilms detected at 24-hour intervals up to 72 hours from initial exposure. (c) Immunohistological staining of C. trachomatis inclusions visualized in HeLa cell monolayers by fluorescence microscopy (400x magnification).
