Figure 1.

Resveratrol blocks and down-modulates TNF-β- and TNF-α-induced proliferation of CRC cells. HCT116 cells in alginate bead cultures were treated as described in Materials and Methods. (a) Light microscopic demonstration of HCT116 cells grown in alginate beads culture (*) after 10 days. The number of colonospheres (arrows) was quantified by counting 15 different microscopic fields. Magnification ×24, bar = 0.2 mm in all cases. (b) The MTT method was used to investigate cell proliferation of HCT116 cells as described in detail in Materials and methods. All experiments were performed at least three times. P < 0.05 (*) and P < 0.01 (**) indicate a significant difference compared to the control group. (A color version of this figure is available in the online journal.)