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. 2019 Feb 5;24:4. doi: 10.1186/s11658-018-0129-6

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression levels of miR-219-5p were downregulated in ESCC tissues and cell lines. a A comparison of miR-219-5p expression levels between 20 paired ESCC tissues (P1–P20) and corresponding non-tumor (NT) tissues. MiR-219-5p expression was normalized to U6, calculated using the 2^-ΔΔCt method, and then compared with miR-219-5p expression in NT tissues. b Quantitative analysis of miR-219-5p levels determined using real-time PCR in four ESCC cell lines (ECA109, KYSE150, EC9706, TE-9) and one normal esophageal cell line (Het-1A). The data are presented as the means ± standard deviations. Experiments were performed in triplicate and repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001; miR, microRNA; ESCC, esophageal squamous cell carcinoma