Fig. 2.

Upregulation of miR-219-5p expression inhibited cell proliferation and colon formation in ESCC. a The expression levels of miR-219-5p in EC9706 and TE-9 cells transfected with miR-219-5p or miR-NC were analyzed using real-time PCR. b Proliferation ability was tested using the MTT method in EC9706 and TE-9 cells transfected with miR-219-5p or miR-NC. c Representative micrographs of colony formation. d Relative quantification of the colonies of the indicated cells. The data are presented as means ± SD of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, compared with miR-NC