Figure 4.

Deletion of Kir4.1 abolishes the effect of dietary Na⁺ intake on NCC. (A) Immunoblot showing the expression of pNCC (at Thr⁵³) and tNCC in the WT (left six lanes) and in Ks-Kir4.1 KO mice (right six lanes) on different Na⁺ diets for 7 days. A bar graph summarizes the normalized band intensity of the above experiments for (B) pNCC and (C) tNCC, respectively (seven mice). Western blots were generated from the tissue lysates harvested from renal cortex. Two-way ANOVA was used for determination of significance.