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. 2019 Jan 8;30(2):277–292. doi: 10.1681/ASN.2018080815

Figure 1.

Figure 1.

β2m-dependent MHC1b, but not MHC II or MHC1a, molecules significantly reduce relative and absolute number of kidney DN T cells. Lymphocytes from kidneys of age-matched WT, β2m KO, MHC II KO, or KD KO mice were isolated, stained, and acquired by LSRII and CD4, CD8, and DN αβ T cell subsets were gated as described (Supplemental Figure 1). (A) Dot plots show representative percentages of CD4, CD8, and DN T cells in kidneys of WT, β2m KO, and MHC II KO mice. Numbers in quadrants indicate percentages. Graphs show cumulative frequency and absolute numbers of T cells in kidney. Data points (mean±SEM) are from at least seven independent experiments (n=7 mice). (B) Dot plots show representative percentages of CD4, CD8, and DN T cells in kidneys of WT and KD KO mice. Numbers in quadrants indicate percentages. Graphs show cumulative frequency, absolute numbers, and percentage of CD69 and CD62L of T cells in kidneys. Data points (mean±SEM) are from at least five independent experiments (n=5 mice). *P<0.05; **P<0.01; ***P<0.001.