Figure 4.

Adoptive transfer of WT CD8 T cells into β2m KO mice significantly increases absolute numbers and activates endogenous DN T cells. Purified CD8 T cells from WT spleens were intraperitoneally injected (5×10⁶ cells per mouse) into β2m KO mice, whereas age-matched WT controls received PBS. Five days later, the frequency and absolute numbers of CD4 and DN T cells in kidneys were analyzed by flow cytometry. Representative dot plots show percentages of CD4, CD8, and DN T cells in kidneys of WT control, β2m KO, and β2m KO mice injected with CD8 T cells [β2m KO (+CD8)]. Numbers in quadrants indicate percentages. Top graphs show cumulative percentages and absolute numbers of each subsets in indicated organs of mice in each group. Bottom graphs show frequency of CD69 and CD62L on gated CD4 and DN T cell subsets in kidneys of mice in the three different groups. Data points expressed as mean±SEM are from at least three independent experiments (n=3 mice). *P<0.05; **P<0.01; ***P<0.001; ****P<0.001.