Figure 7.

β2m-independent PD-1⁺ DN T cell subset is the major responder to ischemic AKI whereas NK1.1⁺ DN T cell subset and CD8 T cells are dispensable. WT and β2m KO mice were subjected to IRI and serum creatinine levels were determined after 24 hours to confirm kidney injury. Lymphocytes were isolated from kidneys of WT, β2m KO, WT-IRI, and β2m KO-IRI mice and the frequency, absolute numbers, and percentage of PD-1⁺ or NK1.1⁺ subsets were analyzed by flow cytometry. (A) Representative dot plots show percentage of PD-1⁺ or NK1.1⁺ by gated T cell subsets in kidneys. Numbers in quadrants indicate percentages. Graph shows cumulative data. (B) Representative dot plots show T cell subsets from a kidney. Numbers in quadrants indicate percentages. Graphs show percentage and absolute numbers of T cells from kidneys from indicated mouse groups. Data points (mean±SEM) are from at least five independent experiments (n=5 mice). *P<0.05; **P<0.01; ***P<0.001.