Figure 2.

Sulforaphane (SFN) inhibited H₂O₂-induced ROS over expression and apoptosis in human trabecular meshwork cells (HTMCs). HTMCs were treated with H₂O₂ (1 μM, 10 μM, 100 μM, and 200 μM) for 24 hours with or without pretreatment of SFN (10 μM, 20 μM, and 30 μM) for 6 hours. (A) The levels of intercellular ROS were detected by using a ROS assay kit. (B) Apoptotic cells were tested by a FITC Annexin V apoptosis detection kit. (C) Protein expression of PARP, Bcl2, and Bax. Data were expressed as mean ± standard deviation. * P<0.05, ** P<0.01. H₂O₂ – hydrogen peroxide; ROS – reactive oxygen species